1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)]-2-(2′-amino-5′-methylphenoxy)ethane-N,N,N′,N′-tetraacetic Acid Pentaacetoxymethyl Ester Reddish orange solid. PROTECT FROM LIGHT. PACKAGED UNDER INERT GAS. Membrane-permeable version of FLUO 3 (Cat. No. 343244). Unlike all other Ca2+ indicators, FLUO 3/AM is non-fluorescent until it is hydrolyzed in the cell by cellular esterases. It is insensitive to Mg2+. Purity: ≥85% by HPLC. Excitation max.: 506 nm, Emission max.: 526 nm (after hydrolysis) CAS 121714-22-5. Ref.: Caputo, C., and Bolanos, P. 1994. J. Physiol. (London) 481, 119. Schumann, M.A., et al. 1993. J. Biol. Chem. 268, 2134. Eberhard, M., and Erne, P. 1989. Biochem. Biophys. Res. Commun. 163, 309. |
