1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)]-2-(2′-amino-5′-methylphenoxy)ethane-N,N,N′,N′-tetraacetic Acid, 5NH4 Orange red solid. PROTECT FROM LIGHT. HYGROSCOPIC. Almost non-fluorescent in the absence of Ca2+, but fluorescence increases 40-fold upon Ca2+ binding. Operates at visible excitation and emission wavelengths. Weaker binding of Ca2+ facilitates measurement of higher peaks of Ca2+ transients than is possible with FURA 2. FLUO 3 is rapidly inactivated by hydroxyl radicals. Has a much larger fluorescence response upon Ca2+ mobilization than FURA 2. Purity: ≥90% by HPLC. Excitation max.: 506 nm, Emission max.: 526 nm CAS 134907-84-9. Ref.: Sarvazyan, N., et al. 1998. Arch. Biochem. Biophys. 350, 132. Okamoto, Y., et al. 1995. Biochem. J. 305, 1011. Novak, E.J., and Rabinovitch, P.S. 1994. Cytometry 17, 135. O’Malley, D.M., et al. 1994. J. Neurosci. 14, 5741. Schuman, M.A., et al. 1993. J. Biol. Chem. 268, 2134. Zucker, R.S. 1992. Cell Calcium 13, 29. Cornell-Bell, A.H., et al. 1990. Science 247, 470. Merritt, J.E., et al. 1990. Biochem. J. 269, 513. Rijkers, G.T., et al. 1990. Cytometry 11, 923. Saavedra-Molina, A., et al. 1990. Biochem. Biophys. Res. Commun. 167, 148. Eberhard, M., and Erne, P. 1989. Biochem. Biophys. Res. Commun. 163, 30. Minta, A., et al. 1989. J. Biol. Chem. 264, 8171. Tsien, R.Y. 1988. Trends Neurochem. Sci. 11, 419. |
