Fr ll Clear yellow liquid. In 25 mM HEPES, pH 7.6. AVOID FREEZE/THAW CYCLES. Protein fraction from rabbit reticulocytes that binds to anion exchange resins. Contains E1, E2s, E3s, PA28, 20S and 26S proteasomes, and ubiquitin C-terminal hydrolases (UCHs). Useful for demonstrating ubiquitin and/or ATP-dependent degradation of protein substrates in vitro, as well as for measurement of activity of protein conjugates in vitro in the presence of proteasome inhibitors and Ubiquitin Aldehyde (Cat. No. 662056). Contaminants: ATP and ubiquitin: none detected. Ref.: Hershko, A., et al. 1986. J. Biol. Chem. 261, 11992. Hershko, A., et al. 1983. J. Biol. Chem. 258, 8206. |
