1-[2-(5-Carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxy]-2-(2′-amino-5′-methylphenoxy)-ethane-N,N,N′,N′-tetraacetic Acid, 5Na Yellow solid. PROTECT FROM LIGHT. HYGROSCOPIC. PACKAGED UNDER INERT GAS. Most widely used ratiometric calcium indicator. Highly selective for Ca2+, however, it does bind other divalent and trivalent cations. Useful for fluorescence microscopy. Binding of Ca2+ to FURA 2 results in a shift in its absorption spectra to shorter wavelengths with little change in emission spectra. Purity: ≥95% by HPLC. Excitation max.: 362 nm (low), 335 nm (high), Emission max.: 512 nm (low), 505 nm (high) Ref.: Arkle, S., et al. 1994. Exp. Physiol. 79, 975. Bals, S., et al. 1990. Cell Calcium 11, 385; Goldman, W.F., et al. 1990. Cell Calcium 11, 221. Hisayama, T., et al. 1990. Br. J. Pharmacol. 100, 677. Ozaki, H., et al. 1988. J. Biol. Chem. 263, 14074. Grynkiewicz, G., et al. 1985. J. Biol. Chem. 260, 3440. Tsien, R.Y., et al. 1985. Cell Calcium 6, 145. |
