Crystalline solid. Aminoglycoside related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis. Toxic to bacteria, yeast, protozoans, helminths, higher plant, and mammalian cells. Widely used in the selection of eukaryotic expression vectors carrying the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3′-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418. Biological activity: Potency: ≥730 µg/mg. Purity: ≥98% by TLC. Also available as a sterile-filtered solution (Cat. No. 345812). pH 4.0 -6.0. RTECS CB9378500, CAS 108321-42-2. Ref.: Ethier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189. Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245. Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY. Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46. Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8. Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166. Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356. Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031. Jimenez, A., and Davies, J. 1980. Nature 287, 869. |
