AR, Human, Recombinant
Dihydrotestosterone Receptor, Human, Recombinant

Liquid. In 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 200 µM EDTA, 20% glycerol, pH 8.0. Recombinant, human androgen receptor (AR) fused at the N-terminus to a Flag-Tag™ sequence and at the C-terminus to His•Tag^® sequence, expressed in S. frugiperda insect cells expressed using a baculovirus expression system, and purified by affinity chromatography. The activity of androgen is mediated by the binding of the intracellular androgen receptor which, when activated, translocates to the nucleus and modulates specific gene transcription by interaction with cis-acting hormone response elements in the target genes. Activity: 1.0 unit is equivalent to 1 ng protein. 20 units are sufficient for a gel-mobility shift assay and 100 units are sufficient for a protein-protein interaction assay.. One unit is defined as 1 ng purified protein.

Ref.: Chen, G., et al. 2005. Prostate 63, 395. Estebanez-Perpina, E., et al. 2005, J. Biol. Chem. 280, 8060. Gao, S., et al. 2005. Mol. Endocrinol. 19, 1792. Hillmer, A.M., et al. 2005. Am. J. Hum. Genet. 77, 140. Kinoshita, M., et al. 2005, Int. J. Urol. 12, 390. Lim, J.T., et al. 2005. Proc. Natl. Acad. Sci. U.S.A. 102, 5156; Marques, R.B., et al. 2005. Int. J. Cancer 117, 221; Metzger, E., et al. 2005. Nature 437, 436; Murthy, S., et al. 2005. Prostate 64, 362; Chang, C., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5946.