Polo-like kinase 2, Human, Recombinant Liquid. In 100 mM NaCl, 25 mM Tris-HCl, 3 mM DTT, 50% glycerol, 0.05% Tween®-20 detergent, pH 8.0. AVOID FREEZE/THAW CYCLES. Full length, recombinant, human Plk2 fused at the N-terminus to GST and expressed in S. frugiperda insect cells using a baculovirus expression system. Plk2 is a G1 specific kinase that is specifically detected in brain, heart, and lung. Plk2 expression increases in the presence of DNA-damaging agents. The active kinase is useful for the study of Plk2 regulation and inhibitor screening. One unit is defined as the amount of enzyme that will phosphorylate 1 pmol SER/THR 16 substrate per min at 30°C, pH 7.5. Purity: ≥70% by SDS-PAGE. Ref.: Eckerdt, F., et al. 2005. Oncogene 24, 267. Warnke, S., et al. 2004.Curr Biol. 14, 1200.
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