Polo-like kinase 3, Human, Recombinant

Liquid. In 100 mM NaCl, 25 mM Tris-HCl, 3 mM DTT, 50% glycerol, 0.05% TWEEN^®-20 detergent, pH 8.0. AVOID FREEZE/THAW CYCLES. Recombinant, human Plk3 consisting of amino acids 58-339 fused at the N-terminus to a His•Tag^® sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. Plk3 is an NF-kB-regulated kinase that mediates p53-dependent and -independent pro-apoptotic responses to elevated levels of superoxide. The active kinase is useful in the study of Plk3 regulation and for inhibitor screening. Specific activity: ≥500 units/µg protein. One unit is defined as the amount of enzyme that is required to phosphorylate 1 nmol casein per min at 30°C, pH 7.4. Purity: ≥80% by SDS-PAGE.

Ref.: Li. Z., et al. 2005. J. Biol. Chem. 280, 16843. Xie, S., et al. 2004. Oncogene 23, 3822.
S: 26-45