Format: 96-well plate

Form: 96 Tests

Detection Method: Colorimetric

Assay Range: 0 - 16 µM for GSH or 0 - 8 µM for GSSG

Assay Time: 3 h

Sample Type: Plasma, serum, erythrocyte lysate, tissue samples, and cultured cells

Positive Control: GSSG

Kit Contents: 2X MES Buffer, GSSG Standard, Cofactor Mixture, Enzyme Mixture, DTNB, 96-Well Plate, Plate Cover, and a user protocol. Assay requires Metaphosphoric Acid and Triethanolamine to be supplied by the user.

Comments: A sensitive and convenient colorimetric (414 or 405 nm) assay kit for the measurement of glutathione from plasma, serum, erythrocyte lysate, tissue samples, and cultured cells. Dynamic range: 0 - 16 µM for GSH or 0 - 8 µM for GSSG. For plasma and serum samples concentration and deproteination are required.

Ref.: Foyer, C.H., et al. 1994. Physiol. Plant. 92, 696. Baillie, T.A. and Slatter, J.G. 1991. Acc. Chem. Res. 24, 264. Baker, M.A., et al. 1990. Anal. Biochem. 190, 360. Inoue, M., et al. 1987. Journal of Protein Chemistry 6, 207. Eyer, P. and Podhradsky, D. 1986. Anal. Biochem. 153, 57. Inoue, M. 1985. in Renal Biochemistry. Kinne, R.K.H. editor. Elsevier Science Publishers B.V. London, 225. Lash, L.H. and Jones, D.P. 1985. Arch. Biochem. Biophys. 240, 583. Griffith, O.W. 1980. Anal. Biochem. 106, 207. Wendel, A. and Cikryt, P. 1980. FEBS Lett. 120, 209. Glutathione: Metabolism and function, Arias, I.M. and Jakoby, W.B. 1976. editors. Raven Press, New York. Tietze, F. 1969. Anal. Biochem. 27, 502.