GSK 3b-Isozyme, Rabbit Skeletal Muscle, Recombinant, E. coli

Liquid. In 50 mM NaCl, 30 mM Tris-HCl, 5 mM DTT, 1 mM EDTA, 50% glycerol, 0.03% BRIJ^®-35 Detergent, pH 7.5 AVOID FREEZE/THAW CYCLES. Recombinant, rabbit glycogen synthase kinase 3b expressed in E. coli. Dual specificity kinase. One of several protein kinases that phosphorylates glycogen synthase. Other substrates include p90^rsk, Tau, c-Jun, and CREB. Plays a key role in Wnt and insulin signaling. Activity: ≥250,000 units/ml. Specific activity: ≥5,000,000 units/mg protein. One unit is defined as the amount of GSK-3 required to catalyze the transfer of 1 pmol phosphate to CREB phosphopeptide KRREILSRRPS(P)YRP (200 µM), in 1 min at 30°C in a reaction volume of 25 µl. Contaminants: DNase: <10% conversion of RFI DNA to RFII and linear forms. Phosphatases and Proteases: none detected. RNases: <10% RNA degradation.. Note: 1 KU = 1000 units.

Ref.: Dajani, R., et al. 2001. Cell 105, 721. Wang, Q.M., et al. 1995. Biochem. Biophys. Res. Commun. 208, 796. Fiol, C.J., et al. 1994. J. Biol. Chem. 269, 32187. Wang, Q.M., et al. 1994. J. Biol. Chem. 269, 14566. Wang, Q.M., et al. 1994. Biochemistry 33, 143. deGroot, R.P., et al. 1993. Oncogene 8, 841. Ishiguro, K., et al. 1993. FEBS Lett. 325, 167.