BIO
(2′Z,3′E)-6-Bromoindirubin-3′-oxime Purple solid. PROTECT FROM LIGHT. PACKAGED UNDER INERT GAS. A cell-permeable bis-indolo (indirubin) compound that acts as a highly potent, selective, reversible, and ATP-competitive inhibitor of GSK-3a/b (IC50 = 5 nM). Its specificity has been tested against various Cdk's (IC50 = 83, 300, 320, and 10,000 nM for Cdk5/p25, Cdk2/A, Cdk1/B, and Cdk4/D1, respectively) as well as many other commonly studied kinases (IC50 ≥ 10 µM), including MAP kinases, PKA, PKC isoforms, PKG, CK, and IRTK. Inhibition of GSK by BIO has been shown to result in the activation of Wnt-signaling pathway and sustained pluripotency in human and murine ESCs (embryonic stem cells). Reported to maintain self-renewal in human and mouse embryonic stem cells. Also induces the differentiation of neonatal cardiomyocytes. Purity: ≥97% by HPLC. CAS 667463-62-9. Ref.: Tseng, A-S., et al. 2006. Chem. Biol. 13, 957. Polychronopoulos, P., et al. 2004. J. Med. Chem. 47, 935. Sato, N., et al. 2004. Nat. Med. 10, 55. Meijer, L., et al. 2003. Chem. Biol. 10, 1255. |
