Assay Time: 3 h-5 days

Kit Contents: Bovine Fetuin Control, Denaturation Solution, Endo-a-N-Acetylgalactosaminidase, b1,4-Galactosidase, Glucosaminidase, N-Glycosidase F, Neuraminidase, Reaction Buffer, TRITON® X-100 Detergent, and a user protocol.

Comments: Kit contains all enzymes and reagents needed to remove all N-linked, all simple O-linked, and virtually all-complex O-linked oligosaccharides from glycoproteins in a single reaction at neutral pH. Suitable for use with native or denaturation protocols, with no degradation of protein. This kit has been assembled to contain sequencing grade enzymes without azide, BSA, or glycerol. Each kit can be used to deglycosylate up to 2 mg glycoprotein.

Ref.: Chiba, A., et al. 1997. J. Biol. Chem. 272, 2156. Jiang, M.S., and Hart, G.W. 1997. J. Biol. Chem. 272, 2421. Wilkins, P.P., et al. 1996. J. Biol. Chem. 271, 18732. Altmann, F., et al. 1995. Glycoconj. J. 12, 84. Szkudlinski, M.W., et al. 1995. Endocrinology 136, 3325. Pahlsson, P., et al. 1994. Glycoconj. J. 11, 43. Saito, S., et al. 1994. J. Biol. Chem. 269, 5644. Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44. Iwase, H., Hotta, K. 1993. Methods. Mol. Biol. 14, 151. Stults, N.L., and Cummings, R.D. 1993. Glycobiology 3, 589. Hard, K., et al. 1992. Eur. J. Biochem. 205, 785. Kuraya, N., and Hase, S. 1992. J. Biochem. (Tokyo) 112, 122. Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646. Fukuda, M., et al. 1987. J. Biol. Chem. 262, 11952. Sojar, H.T., and Bahl, O.P. 1987. Arch. Biochem. Biophys. 259, 52. Taga, E.M., et al. 1984. Biochemistry 23, 815. Kobata, A. 1979. Anal. Biochem. 100, 1. Uchida, Y., et al. 1979. J. Biochem (Tokyo) 86, 1573. Glasgow, L.R., et al. 1977. J. Biol. Chem. 252, 8615. Spiro, R.G., and Bhoyroo, V.D. 1974. J. Biol. Chem. 249, 5704.

R: 20/22-24-36/37/38-41-42/43; S: 26-36/37/39-45-23