GzmA

Liquid. In 520 mM NaCl, 50 mM MES, 10% glycerol, pH 6.0. AVOID FREEZE/THAW CYCLES. Recombinant, human granzyme A expressed in E. coli. Granzyme A is a trypsin-like serine protease secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. A proapoptotic enzyme that induces cell death through caspase-independent mechanism, causing single-stranded DNA nicking and other apoptotic features including nuclear fragmentation, chromatin condensation, loss of mitochondrial potential, and membrane blebbing. Granzyme A is also known to bind to and cleave nucleolin and activate the thrombin receptor on neuronal cells and astrocytes. It also cleaves and activates interleukin-1b. Note: The M.W. is that of a homodimer. Specific activity: ≥100 units/µg protein. One unit is defined as the amount of enzyme that hydrolyzes 1 nmol Z-Lys-SBzl per min 25°C, pH 8.0. EC 3.4.21.78.

Ref.: Fan, Z., et al. 2002. Mol. Cell. Biol. 22, 2810. Russell, J.H., and Ley, T. J. 2002. Annu. Rev. Immunol. 20, 323. Beresford, P.J., et al. 2001. J. Biol. Chem. 276, 43285. Simon, M.M., and Kramer, M.D. 1994. Methods Enzymol. 244, 68.