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Stem Cell Resource

Molecular Biology
  Bisbenzimide H 33342 Fluorochrome, Trihydrochloride
Cat. No. 382065  
All Categories » Calbiochem » Biochemicals » Dyes, Stains and Probes » DNA-Binding Dyes

2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole, 3HCl
HOE 33342

Yellow solid. PROTECT FROM LIGHT. Cell-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei for fluorescence microscopy and flow cytometry applications. Useful for the simultaneous study of the cell cycle and apoptosis in unfixed cells. Induces cell differentiation. H 33342 and the related compound H 33258 (Cat. No. 382061) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs. Exhibits a 10-fold greater cell-permeability than H 33258. Purity: ≥95% by TLC. Excitation max.: 346 nm, Emission max.: 460 nm RTECS DT4200000, CAS 23491-52-3.

Ref.: Werstuck, G., and Green, M.R. 1998. Science 282, 296. Paddenberg, R., et al. 1996. Eur. J. Cell Biol. 71, 105. Filatov, M.V., et al. 1995. Mutat. Res. 327, 209. Belloc, F., et al. 1994. Cytometry 17, 59. Crissman, H.A. 1990. Methods Cell Biol. 33, 97. Hinkley, R.E., et al. 1986. Dev. Biol. 118, 148. Sandhu, L.C., et al. 1985. Cytometry 6, 191. Frankfurt, O.S. 1983. Cytometry 4, 216. Lalande, M.E., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 363. Visser, J.W.M. 1980. Flow Cytometry IV, 86.
R: 23/24/25-36/37/38; S: 26-36/37/39-45

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382065
Hazardous MaterialsAttention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

SolubilityMolecular FormulaMol. Wt.
H2O. Precipitates in phosphate buffered solutions C27H28N6O · 3HCl 561.9 


Alternate Products:
382061: Bisbenzimide H 33258 Fluorochrome, Trihydrochloride
382065: Bisbenzimide H 33342 Fluorochrome, Trihydrochloride

Related Literature:

Caspases and other Apoptosis Related Tools Brochure

Tools and Tips for Analyzing Apoptosis

Material Safety Data Sheets:
382065: Bisbenzimide H 33342 Fluorochrome, Trihydrochloride - English
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Related Categories:
All Categories » Calbiochem » Biochemicals » Dyes, Stains and Probes » DNA-Binding Dyes