2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole, 3HCl HOE 33342 Yellow solid. PROTECT FROM LIGHT. Cell-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei for fluorescence microscopy and flow cytometry applications. Useful for the simultaneous study of the cell cycle and apoptosis in unfixed cells. Induces cell differentiation. H 33342 and the related compound H 33258 (Cat. No. 382061) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs. Exhibits a 10-fold greater cell-permeability than H 33258. Purity: ≥95% by TLC. Excitation max.: 346 nm, Emission max.: 460 nm RTECS DT4200000, CAS 23491-52-3. Ref.: Werstuck, G., and Green, M.R. 1998. Science 282, 296. Paddenberg, R., et al. 1996. Eur. J. Cell Biol. 71, 105. Filatov, M.V., et al. 1995. Mutat. Res. 327, 209. Belloc, F., et al. 1994. Cytometry 17, 59. Crissman, H.A. 1990. Methods Cell Biol. 33, 97. Hinkley, R.E., et al. 1986. Dev. Biol. 118, 148. Sandhu, L.C., et al. 1985. Cytometry 6, 191. Frankfurt, O.S. 1983. Cytometry 4, 216. Lalande, M.E., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 363. Visser, J.W.M. 1980. Flow Cytometry IV, 86. R: 23/24/25-36/37/38; S: 26-36/37/39-45 |