HDAC3
HDAC3/NcoR2
HDAC3/SMRT

Liquid. In 138 mM NaCl, 25 mM Tris-HCl, 0.05% TWEEN^®-20 detergent, 10% glycerol, pH 8.0. AVOID FREEZE/THAW CYCLES. Full-length, recombinant, human histone deacetylase 3 (HDAC3) fused at the C-terminus to a His•Tag^® sequence and human SMRT (NCOR2) consisting of amino acids 395-489 fused at the N-terminus to GST and co-expressed in S. frugiperda insect cells using a baculovirus expression system. SMRT co-expression is required for full HDAC3 activity. The HDAC3-SMRT repression complex represents a novel and potentially specific therapeutic target. The purified complex is useful for the study of HDAC3 regulation and for inhibitor screening. Specific activity: ≥110 units/µg protein. One unit is defined as the amount of enyme that is required to deacetylate 1 pmol fluorescent peptide substrate [Arg-His-Lys-Lys(Ac)], per min at 37°C. Purity: ≥95% by SDS-PAGE. MW: 49700 (HDAC3) and 39000 (SMRT)

Ref.: Guenther, M.G., et al. 2000. Genes Dev. 14, 1048. Li, J., et al. 2000. EMBO J. 19, 4342.