HDAC8, Human, Recombinant

Liquid. In 138 mM NaCl, 25 mM Tris-HCl, 10% glycerol, 0.05% TWEEN^®-20 detergent, pH 8.0. AVOID FREEZE/THAW CYCLES. Full length, recombinant, human histone deacetylase 8 (HDAC8) fused to at the C-terminus to a His•Tag^® sequence at and expressed in S. frugiperda insect cells using a baculovirus expression system. HDAC8 phosphorylated in vivo by PKA results in decreased activity, which leads to the hyperacetylation of histones H3 and H4. HDAC8 may play a role in one of the most frequent types of acute myeloid leukemia (AML). The active deacetylase is useful in the study of HDAC8 regulation and for inhibitor screening. Specific activity: ≥10 units/µg protein. One unit is defined as the the amount of enzyme required to deacetylate 1 picomol fluorescent peptide substrate [Arg-His-Lys(Ac)-Lys(Ac)] per min at 30°C. Purity: ≥90% by SDS-PAGE.

Ref.: Waltregny, D., et al. 2005. FASEB J. 19, 966. Buggy, J.J., et al. 2000. Biochem J. 350, 199