Liquid. In 150 mM NaCl, 50 mM Tris-HCl, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. AVOID FREEZE/THAW CYCLES. Full-length, recombinant, human IkBa fused at the N-terninus to GST and expressed in E. coli. IkBa binds to NF-kB and targets the complex for degradation via the ubiqutin proteasome pathway. The enzyme is useful for binding studies, and for inhibitor and activator assays of NF-kB. Purity: ≥80% by SDS-PAGE. Ref.: Bondeson J, et al. 2007 J Rheumatol. 34,523. Auphan N, et al. 1995.Science.270, 286. |
