Ac-YVAD-pNA
IL-1b Converting Enzyme (ICE) Colorimetric Substrate Lyophilized. PROTECT FROM LIGHT. PROTECT FROM MOISTURE. Colorimetric caspase-1 substrate that is similar in sequence to Caspase-1 Substrate III, Fluorogenic (Cat. No. 400020). Cleavage of pNA is monitored colorimetrically at ~405 nm (e = 9,160 M-1cm-1). Sequence is based on the caspase-1 cleavage site of the IL-1b precursor and includes Asp116. Also a substrate for caspase-4. Purity: ≥95% by HPLC. Sold on the basis of peptide content. Ac-Tyr-Val-Ala-Asp-pNA Ref.: Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312. Reiter, L.A. 1994. Int. J. Pept. Protein Res. 43, 87. Thornberry, N.A., et al. 1994. Methods Enzymol. 244, 615. Thornberry, N.A., et al. 1992. Nature 356, 768. |
