Caramel-colored liquid. Unique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells. Inhibits protein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA. Hph, a gene from E. coli, encodes resistance to hygromycin B and can be isolated and cloned by recombinant DNA techniques. This hygromycin B-resistance gene is particularly useful for identification or selection of recombinant clones in a variety of cell types. Hygromycin B penetrates cells that have been permeabilized by virus infection and can act as an effective antiviral agent. Biological activity: The activity of Hygromycin B is measured in a zonal inhibition assay using Bacillus subtilis. The activity is measured relative to a reference standard.. Purity: >80% by HPLC. Bioassay: 350-450 units/mg Hygromycin B. 10⁶ units is equivalent to ~900 mg. RTECS WK2130000, CAS 31282-04-9. Supplied with a directional insert. Note: 1 KU = 1000 units; 1 MU = 1,000,000 units.

Merck Index: 14, 4852
Ref.: Dean, N. 1995. Proc. Natl. Acad. Sci. USA 92, 1287. Hamada, W., et al. 1994. Curr. Genetics 26, 251. Hubbard, S. C., et al. 1994. J. Biol. Chem. 269, 3717. Rikkerink, E. H., et al. 1994. Current Genetics 25, 202. Sugimoto, K., et al. 1994. Plant J. 5, 863. Buchschacher, G.L., Jr., and Panganiban, A.T. 1992. J. Virol. 66, 2731. Gaken, J., et al. 1992. Biotechniques 13, 32. Lama, J., and Carrasco, L. 1992. J. Biol. Chem. 267, 15932. Ma, H., et al. 1992. Gene 117, 161. MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol. 276, 67. Norman, J.A., et al. 1992. Mol. Pharmacol. 41, 53. Bilang, R., et al. 1991. Gene 100, 247. Dale, E., and Ow, D. 1991. Proc. Natl. Acad. Sci. USA 88, 10558. Lee, M.G-S., and Van der Ploeg, L. 1991. Gene 105, 255. Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol. 57, 1423. MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother. 35, 2125. Zhou, J., et al. 1991. Gene 107, 307. Bulte, L., and Bennoun, P. 1990. Current Genetics 18, 155. Giordano, T.J., and McAllister, W.T. 1990. Gene 88, 285. Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 24, 1915. Carrasco, L., et al. 1989. Pharmacol. Ther. 9, 311. Crespi, C.L., et al. 1989. Carcinogenesis 10, 295. Damm, B., et al. 1989. Mol. Gen. Genetics 217, 6. Egelhoff, T.T., et al. 1989. Mol. Cell. Biol. 9, 1965. Kronstad, J.W., et al. 1989. Gene 79, 97. Perlin, D.S., et al. 1988. J. Biol. Chem. 263, 118. Cullen, D., et al. 1987. Gene 57, 21. Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459. Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun. 117, 6. Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.
R: 26/27/28-37/38-41; S: 23-26-36/37/39-45