IDE
Insulysin

Liquid. In 100 mM potassium phosphate buffer, 20% glycerol, pH 7.3. Recombinant, rat insulin degrading enzyme fused to a His•Tag^® sequence and expressed in S. frugiperda insect cells. A metalloprotease that degrades insulin and a variety of other peptides including amyloid peptides. Specific activity: ≥3 U/mg protein. One unit is defined as the amount of the enzyme that will catalyze the hydrolysis of 1 µmol iodinated insulin per h at 37°C, pH 7.3. Purity: ≥90% by SDS-PAGE. EC 3.4.24.56.

Ref.: Hersh, L.B. 2003. Curr. Pharm. Des. 9, 449. Miller, B.C., et al. 2003. Proc. Natl. Acad. Sci. USA 100, 6221. Song, E.S., et al. 2003. J. Biol. Chem. 278, 49789. Mukherjee, A., and Hersh, L.B. 2002. J. Alzheimers Dis. 4, 341. Song, E.S., et al. 2001. J. Biol. Chem. 276, 1152. Becker, A.B., and Roth, R.A. 1995. Methods Enzymol. 248, 693.