rhIL-1arhIL-1brhIL-10 Lyophilized. Carrier-free. Recombinant, human interleukin-10 expressed in S. frugiperda insect cells. Pleiotropic cytokine that can exert either immunosuppressive or immunostimulatory effects on a variety of cell types. Potent modulator of monocyte/macrophage function. Exhibits anti-inflammatory effects by suppressing macrophage proliferation. Blocks lipopolysaccharide induction of MMP-2 and MMP-9. Known to reduce neuronal degeneration following CNS injury by blocking glutamate-induced caspase-3 activation. Human IL-10 is also active on murine cell lines. Biological activity: ED50 = 0.2-1 ng/ml as measured in a cell proliferation assay using a mouse mast cell line, MC/9.. Purity: ≥95% by SDS-PAGE. Contaminants: Endotoxin: <100 pg/mg IL-10. Ref.: Bachis, A., et al. 2001. J. Neurosci. 21, 3104. Fortunato, S.J., et al. 2001. Obstet. Gynecol. 98, 284. Fleming, S.D., et al. 1996. J. Immunol. 156, 1143. Wang, C.Q., et al. 1996. J. Cell Physiol. 166, 305. Niro, H., et al. 1994. Int. Immunol. 6, 661. Moore, K., et al. 1993. Annu. Rev. Immunol. 11, 165. Vieira, P., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 1172. |
