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| | PhosphoDetect™ Anti-IR/IGF1R (pTyr1158) Rabbit pAb |
|  | Cat. No. 407706 |
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Insulin Receptor/Insulin-Like Growth Factor 1 Receptor, Phospho-Specific (Tyr1158), Human (Rabbit) | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1158 phosphorylation site of human insulin receptor (IR) | | Form: Liquid | | Formulation: In phosphate buffer, 1 mg/ml BSA, pH 7.4. | | Preservative: ≤0.1% sodium azide | | Positive Control: Insulin stimulated CHO-T cells expressing insulin receptor or insulin-stimulated HepG2 cells | | Comments: Recognizes the ~95 kDa b subunit of the IR protein phosphorylated at Tyr1158 in insulin-stimulated HepG2 cells. Cross-reacts with the insulin-like growth factor-1 receptor (IGF-1R) phosphorylated at Tyr1131. | | Ref.: Ottensmeyer, F.P., et al. 2000. Biochemistry 39, 12103. Virkamaki, A., et al. 1999. J. Clin. Invest. 103, 931. Schmid, E., et al. 1998. FASEB J. 12, 863. Hari, J. and Roth, R.A. 1987. J. Biol. Chem. 262, 15341. Morgan, D.O., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 328. White, M.F. and Kahn, C.R. 1986. The Enzymes 17, 247. Ebina, Y., et al. 1985. Cell 40, 747. Ganguly, S., et al. 1985. Current Topics in Cellular Regulation 27, 83. Grunberger, G., et al. 1984. Science 223, 932. |
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Detection of insulin receptor, phosphorylated on Tyr 1158, by immunoblotting. Samples: Whole cell lysate from CHO-T cells transfected with an insulin receptor containing vector and stimulated with insulin (lanes 1-4), or left unstimulated (lane 5). Primary antibody: PhosphoDetect™ Anti-IR/IGF1R (pTyr 1158) Rabbit pAb (Cat. No. 407706) (1:1000) pre-incubated without peptide (lanes 1, 5), with non-phosphorylated peptide corresponding to the immunogen (lane 2), a generic phosphotyrosine-containing peptide (lane 3), or phosphopeptide immunogen (lane 4). Detection: chemiluminescence.
Detection of insulin receptor, phosphorylated on Tyr1158, by immunocytochemistry (IF). Samples: CHO-T cells, serum-starved overnight, left untreated (left panel) or treated with insulin (100 nM, 10 min, right panel), fixed with 4% paraformaldehyde (20 min, RT), and permeabilized in PBS containing 0.1% saponin and 3% BSA (45 min, RT). Primary antibody: PhosphoDetect™ Anti-IR/IGF1R (pTyr1158) Rabbit pAb (Cat. No. 407706) (1:100). Detection: flourescence.
Detection of insulin receptor, phosphorylated on Tyr1158, by immunocytochemistry (IF). Samples: CHO-T cells, serum-starved overnight, treated with insulin (100 nM, 10 min), fixed with 4% paraformaldehyde (20 min, RT), and permeabilized in PBS containing 0.1% saponin and 3% BSA (45 min, RT, all panels). Primary antibody: PhosphoDetect™ Anti-IR/IGF1R (pTyr1158) Rabbit pAb (Cat. No. 407706) (1:100), pre-incubated with non-phosphorylated peptide corresponding to the immunogen (panel A), phosphopeptide immunogen (panel B), non-phosphorylated peptide corresponding to the Tyr1162 phosphorylation site (panel C), or phosphopeptide surrounding the Tyr1162 phosphorylation site (panel D). Detection: fluorescence.
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