| | PhosphoDetect™ Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb | |  | Cat. No. 407707 | |
| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1162/1163 phosphorylation sites of human insulin receptor | | Form: Liquid | | Formulation: In Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3. | | Preservative: ≤0.1% sodium azide | | Comments: Recognizes the ~95 kDa b subunit of the insulin receptor protein phosphorylated at Tyr1162 and Tyr1163 in insulin receptor transfected cells. Cross-reacts with the insulin-like growth factor-1 receptor phosphorylated at Tyr1135 and Tyr1136. | | Ref.: Bevan, P. 2001. J. Cell Sci. 114, 1429. Ottensmeyer, F.P., et al. 2000. Biochemistry 39, 12103. Virkamaki, A., et al. 1999. J. Clin. Invest. 103, 931. Schmid, E., at al. 1998. FASEB J. 12, 863. Hari, J. and Roth, R.A. 1987. J. Biol. Chem. 262, 15341. Morgan, D.O., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 328. White, M.F. and Kahn, C.R. 1986. The Enzymes 17, 247. Ebina, Y., et al. 1985. Cell 40, 747. Ganguly, S., et al. 1985. Current Topics in Cellular Regulation 27, 83. Grunberger, G., et al. 1984. Science 223, 932. | |
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* Additional Product Information
 Detection of insulin receptor, phosphorylated on Tyr 1162/1163, by immunoblotting. Samples: Whole cell lysate from CHO-T cells transfected with an insulin receptor containing vector and left unstimulated (lane 1), or stimulated with insulin (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb (Cat. No. 407707) (1:1000) pre-incubated without peptide (lanes 1, 5), with phosphopeptide immunogen (lane 2), non-phosphorylated peptide corresponding to the phosphopeptide (lane 3), or a generic phosphotyrosine-containing peptide (lane 4). Detection: chemiluminescence.
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