Ipase-T

Liquid. In 150 mM NaCl, 50 mM HEPES, 1 mM DTT, pH 7.6. AVOID FREEZE/THAW CYCLES. Native, rabbit isopeptidase T. A member of the higher molecular weight group of ubiquitin C-terminal hydrolases (UCHs) involved in the hydrolysis of isopeptide linkages of polyUb chains. Ipase-T activity is regulated by ubiquitin levels. Plays a major role in ubiquitin recycling and in the regulation of 26S proteasome activity. Recommended reaction buffer: 25 mM HEPES, 10 mM DTT, 25°C, pH 7.5. Pre-incubate the enzyme for 15 minutes with 10 mM DTT to achieve maximum activity. Biological activity: Fully functional as measured using 5 nM Ipase-T and 1 µM Ub-AMC (Cat. No. 662075). Purity: ≥95% by SDS-PAGE.

Ref.: Dang, L.C., et al. 1998. Biochemistry 37, 1868. Melandri, F.D., et al. 1996. Biochemistry 35, 12893. Stein, R.L., et al. 1995. Biochemistry 34, 12616. Pickart, C.M., and Rose, I.A. 1986. J. Biol. Chem. 261, 10210.