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| | PhosphoDetect™ Anti-JAK1 (pTyr1022/1023) Rabbit pAb |
|  | Cat. No. 420092 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1022 and Tyr1023 phosphorylation sites of human JAK1 (Janus Kinase 1) | | Form: Liquid | | Formulation: In Dulbecco's PBS (without Ca2+ and Mg2+), 1 mg/ml BSA, 50% glycerol, pH 7.3. | | Preservative: ≤0.1% sodium azide | | Positive Control: 3T3L1 cells treated with LIF | | Comments: Recognizes the ~130 kDa phosphorylated JAK1 in LIF-treated 3T3-L1 cells. Does not cross-react with other JAKs or related kinases. | | Ref.: Liu, K.D., et al. 1997. Curr. Biol. 7, 817. Gauzzi, M.C., et al. 1996. J. Biol. Chem. 271, 20494. | | Manufactured by Biosource International. |
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EMD Chemicals Inc. USD list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
| | human, mouse
not rat | FC, IB
See Key |
Detection of mouse JAK1, phosphorylated on Tyr1022/1023, by immunoblotting. Samples: Whole cell lysate from 3T3L1 cells left unstimulated (lane 1) or stimulated with LIF (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-JAK1 (pTyr1022/1023) Rabbit pAb (Cat. No. 420092) (1:1000) pre-incubated without peptide (lanes 1,2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
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