c-Jun NH2-terminal kinase | Format: Immunoblot | | Form: 40 Tests | | Detection Method: Immunoblot | | Species Reactivity: human, mouse, rat | | Sample Type: Cell lysates | | Kit Contents: JNK Extraction Buffer, JNK-Specific Antibody, Protein A Agarose, c-Jun Protein/ATP Mixture, Kinase Assay Buffer, a c-Jun Phospho-Specific Antibody, and a user protocol. | | Comments: Assay designed to detect JNK/SAPK activity in cell lysates. The assay utilizes a JNK-specific antibody to immunoprecipitate JNK from cell lysates, then JNK activity is detected in a kinase reaction using c-Jun as a substrate. Phosphorylation of c-Jun can be analyzed by Western blot using a c-Jun phospho-specific antibody. | | Note: 1 kit = 40 assays |
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