5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine Iodide Red-orange solid. PROTECT FROM LIGHT. PACKAGED UNDER INERT GAS. A cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential DYm in cells, tissues, and isolated mitochondria. Mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates. Ideal for detection of collapse of DYm induced by uncouplers of mitochondrial respiration. JC-1 has also been used in a functional assay of multidrug-resistance cells and in apoptosis-related mitochondrial modifications. Purity: ≥95% by HPLC. CAS 47729-63-5. Ref.: Mathur, A., et al. 2000. Cardiovasc. Res. 46, 126. Gravance, C.G., et al. 2000. Theriogenology 53, 1691. Chanda, B., and Mathew, M.K. 1999. Biochim Biophys. Acta 1416, 92. Dedov, V.N., and Roufogalis, B.D. 1999. FEBS Lett. 456, 171. Polla, B.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 6458. White, R.J., and Reynolds, I.J. 1996. J. Neurosci. 16, 5688. Ankacrona, M., et al. 1995. Neuron 15, 961. Reers, M., et al. 1995. Methods Enzymol. 260, 406. Cossarizza, A., et al. 1993. Biochem. Biophys. Res. Commun. 197, 40.
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