hSRP1a
Importin-a2
Rch1 Liquid. In 100 mM potassium acetate, 20 mM HEPES-KOH, 1 mM DTT, pH 7.3. AVOID FREEZE/THAW CYCLES. Receptor for the classical nuclear localization sequence (NLS) that acts as an adapter between the NLS and karyopherin b. The NLS/karyopherin a/b trimer, in combination with Ran and an energy source, supports the nuclear import of NLS-containing proteins. Suitable for use in nuclear import and binding studies. Biological activity: maximal import of a rhodamine labeled BSA-NLS substrate in digitonin-permeabilized HeLa cells with ≤0.25 µM karyopherin-a2 added to the import mix. Purity: ≥90% by SDS-PAGE. Ref.: Gruss, O.J., et al. 2001. Cell 104, 83. Connolly, M.D., et al. 2000. Bioorg. Med. Chem. Lett. 10, 951. Park, S.B., et al. 2000. Bioorg. Med. Chem. Lett. 10, 955. Schwoebel, E.D., et al. 1998. J. Biol. Chem. 273, 35170. Weis, K., et al. 1995. Science 268, 1049. Görlich, D., et al. 1994. Cell 79, 767. |
