brmOB Lyophilized. Lyophilized from a sterile filtered PBS. Recombinant, mouse leptin espressed in E. coli. Native leptin is a product of the obese (ob) gene that serves as a ligand for the OB receptor (OB-R). Mice with mutations in the ob gene have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. Suppresses insulin secretion by inhibiting activities of Ca2+-dependent PKC isoforms. Reported to reduce hepatic glucose production by blocking phosphoenolpyruvate synthesis. Biological activity: ED50 = 0.2-1 ng/ml as measured by its ability to induce proliferation of leptin-dependent rOB-R transfected murine BAF3 cells. Purity: ≥97% by SDS-PAGE. Contaminants: Endotoxin: ≤1.0 EU/µg leptin. CAS 181030-10-4. Merck Index: 14, 5443
Ref.: Anderwald, C., et al. 2002. Mol. Endocrinol. 16, 1612. Ookuma, M., et al. 1998. Diabetes 47, 219. Campfield, L.A., et al. 1995. Science 269, 546. Halaas, J.L., et al. 1995. Science 269, 543. Pelleymounter, M.A., et al. 1995. Science 269, 540. Zhang, Y., et al. 1994. Nature 372, 425. |
