rhMIP-1b Lyophilized. Lyophilized from sterile-filtered 30% acetonitrile, 0.1% TFA, 50 µg BSA/µg MIP-1b. Recombinant, human macrophage inflammatory protein-1b expressed in S. frugiperda insect cells. A low molecular weight chemokine involved in inflammation, hematopoiesis, and immunoregulation. Exhibits chemoattractant activity on monocytes and chemoattractant and proadhesive effects on lymphocytes. Biological activity: ED50 = 40-60 ng/ml as measured by its ability to inhibit mouse hematopoietic stem cell proliferation in an in vitro colony assay that detects primitive cells. Purity: ≥97% by SDS-PAGE. Contaminants: Endotoxin: ≤100 pg/µg MIP-1b. Ref.: Taub, D.D., et al. 1993. Science 260, 355. Napolitano, M., et al. 1991. J. Biol. Chem. 266, 17531. Oppenheim, J.J., et al. 1991. Annu. Rev. Immunol. 9, 617. Schall, T.J. 1991. Cytokine 3, 165. Lipes, M.A., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 9704. |
