Collagenase-2
Human Neutrophil Collagenase
Matrix Metalloproteinase 8 Liquid. In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl2, 1 µM ZnCl2, 0.05% BRIJ® 35 Detergent, 0.05% NaN3, pH 7.0. AVOID FREEZE/THAW CYCLES. Native pro-MMP-8 from stimulated human neutrophils. MMP-8 is an N-linked, complex glycoprotein that preferentially hydrolyzes Type I collagen versus Types II and III. Activated MMP-8 is inhibited by TIMP-1. Requires activation just prior to use. MW 58800/44000. Specific activity: ≥40 mU/mg protein. One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C, pH 7.0. Purity: ≥90% by SDS-PAGE. Contaminants: No other MMP activity detectable. Prepared from stimulated neutrophils that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV. EC 3.4.24.34. Note: 1 mU = 1 milliunit. Ref.: Deschamps, A. M., et al. 2005. Circulation 111, 1166. Mallya, S.K., et al. 1990. Biochemistry 29, 10628. Marcy, A.I., et al. 1991. Biochemistry 30, 6476. Stricklin, et al. 1983. Biochemistry 22, 61. |
