DABCYL-GABA-PQGL-E(EDANS)-AK-NH₂
TNO211

Red lyophilized solid. Supplied as a trifluoroacetate salt. PROTECT FROM LIGHT. PACKAGED UNDER INERT GAS. A specific fluorogenic resonance energy transfer (FRET) peptide substrate with an MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination. Useful for the detection of MMP activity [k_cat/K_m = 619,000 M^-1s^-1 for MMP-2, 206,000 M^-1s^-1 for MMP-9, 40,000 M^-1s^-1 for MMP-3, and 21,000 M^-1s^-1 for MMP-1; at 37°C, pH 7.6 ]. Exhibits a high degree of sensitivity that is not affected by optical disturbances in biological media. Also useful for MMP activity measurements in synovial fluid and culture medium. Purity: ≥97% by HPLC. Excitation max.: ~340 nm, Emission max.: ~485 nm

DABCYL-GABA-Pro-Gln-Gly-Leu-Glu(EDANS)-Ala-Lys-NH₂

Ref.: Beekman, B., et al. 1996. FEBS Lett 390, 221.