DABCYL-GABA-RPKPVE-Nva-WR-E(EDANS)-AK-NH₂
TNO003

Red lyophilized solid. Supplied as a trifluoroacetate salt. PROTECT FROM LIGHT. PACKAGED UNDER INERT GAS. A specific fluorescence resonance energy transfer (FRET) peptide substrate for the detection of MMP activity [k_cat/K_m = 29,000 M^-1s^-1 for MMP-3, 13,000 M^-1s^-1 for MMP-13, 7,500 M^-1s^-1 for MMP-9, and 6,000 M^-1s^-1 for MMP-5; at 25°C, pH 7.5]. Substrate cleavage occurs between Glu-Nva (Nva = norvaline). Exhibits a high degree of sensitivity that is not affected by optical disturbances in biological media. Purity: ≥97% by HPLC. Excitation max.: ~360 nm, Emission max.: ~490 nm

DABCYL-GABA-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Glu(EDANS)-Ala-Lys-NH₂ (Nva = Norvaline)

Ref.: Beekman, B., et al. 1997. FEBS Lett 418, 305.