DANSYL-PLA-C(OMeBz)-WAR-NH₂
MT1-MMP Substrate II, Fluorogenic

Yellow lyophilized solid. Supplied as a trifluoroacetate salt. PROTECT FROM LIGHT. PACKAGED UNDER INERT GAS. A specific fluoresence resonance energy transfer (FRET) peptide substrate for the detection of MMP activity. [k_cat/K_m = 1.59 x 10⁶ M^-1s^-1 for MMP-14 (MT-1-MMP) and 1.67 x 10⁴ M^-1s^-1 for MMP-11 (ST3), pH = 7.5 at 25°C; k_cat/K_m = 7.0 x 10⁵ M^-1s^-1 for MMP-14 (MT-1-MMP), 1.5 x 10⁴ M^-1s^-1 for MMP-11, 8.8 x 10³ M^-1s^-1 for MMP-1, and 5.0 x 10³ M^-1s^-1 for MMP-7; pH = 6.8 at 25°C]. In this substrate replacement of leucine by p-OMeBz cysteine increases k_cat/K_m by a factor of 37 for MT1-MMP, and by a factor of 9 for MMP-11. Purity: ≥97% by HPLC. Excitation max.: ~280 nm, Emission max.: ~340 nm

DANSYL-Pro-Leu-Ala-Cys(p-OMeBz)-Trp-Ala-Arg-NH₂ (Bz = Benzyl)

Ref.: Holtz, B., et al. 1999. Biochemistry 38, 12174. Mucha, A., et al. 1998. J. Biol. Chem. 273, 2763.