DNP-GPLGMRGL-NH2 Yellow lyophilized solid. Supplied as a trifluoroacetate salt. PROTECT FROM LIGHT. PACKAGED UNDER INERT GAS. A highly sensitive substrate for assaying MMP activities. Evaluation requires HPLC separation (monitor at l = 350 nm). The substrate cleavage between Gly-Met is determined by HPLC separation (monitored at l = 350 nm). kcat/Km (M-1s-1) at 37°C, pH 7.5, is 4.22 x 106 for MMP-13, 3.9 x 105 for MMP-9, 5.14 x 103 for MMP-1, and 3.14 x 103 for MMP-3. Purity: ≥95% by HPLC. DNP-Gly-Pro-Leu-Gly-Met-Arg-Gly-Leu-NH2 Ref.: Deng, S.J., et al. 2000. J. Biol. Chem. 275, 31422. |
