Format: Flow cytometry or fluorescence microscopy

Form: 100 Tests

Detection Method: Fluorescence

Assay Time: 30-60 min

Sample Type: Cell suspension or adherent cells

Kit Contents: MitoCapture™ Reagent, Incubation Buffer, and a user protocol.

Comments: This kit provides a simple, fluorescent-based method for distinguishing between healthy and apoptotic cells by detecting the changes in the mitochondrial membrane potential. Utilizes MitoCapture™ Reagent, a cationic dye that fluoresces differently in healthy and apoptotic cells. In healthy cells, MitoCapture™ Reagent accumulates and aggregates in the mitochondria, emitting a bright red fluorescence. In apoptotic cells, this reagent cannot aggregate in the mitochondria due to the altered mitochondrial membrane potential, and thus it remains in the cytoplasm in its monomeric form and emits green fluorescence. The fluorescent signals can be easily detected by fluorescent microscopy.

R: 36/37/38; S: 26-36