MPO

Lyophilized. Lyophilized from 100-700 mM NaCl, 50 mM sodium acetate buffer, pH 6.0. Native, human myeloperoxidase from polymorphonuclear leukocytes. A lysosomal enzyme that catalyzes oxidations by hydrogen peroxide, including MPO-chloride-mediated killing of microbes and tumor cells, inactivation of chemotactic factors, and cross-linking of proteins. MPO catalyzes the formation of hypochlorous acid that kills bacteria either by binding the halide ion to intracellular constituents or by oxidation of bacterial lipids and proteins. Specific activity: 150-200 units/mg protein. One unit is defined as the amount of enzyme that will reduce 1.0 µmol H₂O₂ per min at 25°C, pH 6.0. Purity: ≥95% by Electrophoresis. Prepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV. EC 1.11.1.7, CAS 9003-99-0.

Ref.: Nauseef, W.M., et al. 1998. J. Leukocyte Biol. 63, 264. Panasenko, O.M., et al. 1994. Free Radic. Biol. Med. 16, 143. Mulligan, M.S., et al. 1992. Br. J. Pharmacol. 107, 1159. Sharonov, B.P., and Churilova, I.V. 1992. Biochem. Biophys. Res. Commun. 189, 1129.