MCA-RPKPYANvaWMK(DNP)-NH₂

Lyophilized yellow solid. Supplied as a trifluoroacetate salt. PROTECT FROM LIGHT. PROTECT FROM MOISTURE. A quenched fluorescence substrate for MMPs. MMP-3 (stromelysin-1) hydrolyzes this substrate (k_cat/K_m = 5.9 x 10⁴ M^-1sec^-1) at a rate 60 times faster than the hydrolysis by MMP-1 (interstitial collagenase). NFF-2 shows little discrimination between MMP-3, MMP-2 (gelatinase-A; k_cat/K_m = 5 x 10⁴ M^-1s^-1), and MMP-9 (gelatinase-B; k_cat/K_m = 5.5 x 10⁴ M^-1s^-1). Purity: ≥98% by HPLC. Excitation max.: ~325 nm, Emission max.: ~393 nm Supplied as a trifluoroacetate salt.

MCA-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(DNP)-NH₂ (DNP = 2,4-dinitrophenyl; Nva = L-norvaline)

Ref.: Nagase, H., et al. 1994. J. Biol. Chem. 269, 20952.