MCA-RPKPVENvaWRK(DNP)-NH₂

Lyophilized yellow solid. Supplied as a trifluoroacetate salt. PROTECT FROM LIGHT. PROTECT FROM MOISTURE. A quenched fluorescent substrate for MMP-3. This substrate is hydrolyzed rapidly by MMP-3 (stromelysin-1; k_cat/K_m = 2.2 x 10⁵ M^-1s^-1). Hydrolyzed by MMP-9 (gelatinase-B; k_cat/K_m = 1.0 x 10⁴ M^-1s^-1) at a much slower rate. Purity: ≥98% by HPLC. Excitation max.: ~325 nm, Emission max.: ~393 nm

MCA-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(DNP)-NH₂ (DNP = 2,4-dinitrophenyl; Nva = L-norvaline)

Ref.: Nagase, H., et al. 1994. J. Biol. Chem. 269, 20952.