Liquid. In 50 mM L-Arg, 50 mM L-Glu, 50 mM NaCl, 20 mM HEPES, 2 mM DTT, 1 mM EDTA, 50% glycerol, 0.03% BRIJ^® 35 Detergent, pH 7.5. AVOID FREEZE/THAW CYCLES. Full-length, recombinant, human IKKb (amino acids 1-756) (GenBank target symbol= IKBKB, ACC No. NM_001556) fused at the N-terminus with His•Tag^® and S•Tag™ sequences and expressed in S. frugiperda insect cells. IKKb is the principal kinase responsible for phosphorylating IkBa, the regulatory subunit of NF-kB transcription factor. Phosphorylation of IkBa leads to its ubiquitination and proteasome degradation, which allows results in the rapid translocation of NF-kB to the nucleus and transcriptional activation of NF-kB-dependent genes. Specific activity: ≥50 units/mg protein. One unit is defined as the amount of enzyme that will convert 1 nmol ATP to ADP per min per mg protein at 37°C. Purity: ≥80% by SDS-PAGE.

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