Phosphatase and Tensin Homolog Deleted on Chromosome 10 Liquid. In 150 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 1 mM EDTA, 50% glycerol, pH 7.5. Full-length, recombinant, human PTEN (GenBank target symbol = PTEN, ACC No. BC_005821) fused at N-terminus to a His•Tag® and S•Tag™ sequences and expressed in S. frugiperda insect cells. PTEN is a tumor suppressor that is frequently found mutated in human cancers. It serves as a lipid phosphatase and negative regulator of the PI-3K/Akt pathway. Absence of functional PTEN results in constitutive activation of Akt and mTOR kinases. Specific activity: ≥75 U/mg protein. One unit is defined as the amount of enzyme required to liberate 1 nmol phosphate from PIP3 per min at 37°C. Purity: ≥80% by SDS-PAGE. Ref.: Harrington, L.S., et al. 2005. Trends Biochem. Sci. 30, 35. Osaki, M., et al. 2004. Apoptosis 9, 667. Lennon, G., et al. 1996. Genomics 33, 151. |
