Liquid. In 270 mM sucrose, 150 mM NaCl, 50 mM Tris-HCl, 2 mM DTT, 1 mM benzamidine, 0.2 mM PMSF, 0.1 mM EGTA, 0.03% BRIJ^® 35 Detergent, pH 7.5. AVOID FREEZE/THAW CYCLES. Full-length, recombinant, human Aurora A Kinase (amino acids 1-403) (GenBank target symbol= AURKA, ACC No. NM_003600) fused at the N-terminus to His•Tag^® and S•Tag^™ sequences and expressed in S. frugiperda insect cells. Aurora A plays an important role in chromosome segregation and cell division. Specific activity: >500 units/mg protein. One unit is defined as the amount of enzyme that will generate 1.0 nmol ADP from ATP per min per mg protein. Purity: ≥80% by SDS-PAGE.

Ref.: Lennon, G., et al. 1996. Genomics 33, 151.