Nuclear Inhibitor of Protein Phosphatase-1 Liquid. In 500 mM NaCl, 20 mM Tris, 500 µM benzamidine, 500 µM PMSF, 5 µM leupeptin, pH 7.5. AVOID FREEZE/THAW CYCLES. Recombinant, bovine NIPP-1 fused to a His•Tag® sequence and expressed in E. coli. Potent and specific inhibitor of protein phosphatase 1 (PP1; Ki = 1-10 pM) that can be used to distinguish PP1 from other major serine/threonine protein phosphatases including PP2A, PP2B, and PP2C. Also an ideal substrate for protein kinase A and casein kinase II. Thought to be involved in the targeting of PP1 to RNA-associated substrates and may also be involved in the dephosphorylation of transcription factors like CREB and the tumor suppressor Rb. Purity: ≥90% by SDS-PAGE. Ref.: Beullens, M., et al. 1999. J. Biol. Chem. 274, 14053. Jagiello, I., et al. 1997. J. Biol. Chem. 272, 22067. Jagiello, I., et al. 1995. J. Biol. Chem. 270, 17257. Van Eynde, A., et al. 1995. J. Biol. Chem. 270, 28068. Van Eynde, A., et al. 1994. Biochem. J. 297, 447. Beullens, M., et al. 1993. J. Biol. Chem. 268, 13172. Beullens, M., et al. 1992. J. Biol. Chem. 267, 16538. |
