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Oxidative Stress Kits
  Nitric Oxide Assay Kit, Colorimetric
Cat. No. 482650  
All Categories » Calbiochem » Kits and Tools » Nitric Oxide and Nitric Oxide Synthase Assays

Format: 96-well plate
Form: 80 Tests
Detection Method: Colorimetric
Assay Time: 1-1.5 h
Sample Type: Aqueous solutions
Kit Contents: Recombinant Zea mays Nitrate Reductase, NADH, Nitrate Standard, Color Reagents, Buffer, 96-Well Plate, and a user protocol.
Comments: Assay kit for the rapid quantitative measurement of total nitric oxide (NO). Based on the enzymatic conversion of nitrate to nitrite by nitrate reductase, followed by the spectrophotometric quantitation of nitrite levels using Griess Reagent. Do not use with nitrate- or nitrite-containing tissue culture media such as RPMI.
Ref.: Nims, R.W., et al. 1996. Methods Enzymol. 268, 93.
R: 34-36/37/38-40-42/43-20/21/22; S: 26-36-45

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Related Literature:

Nitric Oxide and Oxidative Stress Brochure

Inhibitor Sourcebook

Material Safety Data Sheets:
482650: Nitric Oxide Assay Kit, Colorimetric - English
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All Categories » Calbiochem » Kits and Tools » Nitric Oxide and Nitric Oxide Synthase Assays

Selected Citations:
  1. Carlos Milla, et al. (2004) Myeloperoxidase deficiency enhances inflammation after allogeneic marrow transplantation. American Journal of Physiology Lung Cellular and Molecular Physiology 287, L706-L714.