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 | -20°C |  | Blue ice |  | Hazardous |  | Multiple Toxicity Values, refer to MSDS | | Note: Store and Ship conditions may differ. | | See Key |
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| | Nitric Oxide Assay Kit, Fluorometric |
|  | Cat. No. 482655 |
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| Format: 96-well plate | | Form: 192-288 Tests | | Detection Method: Fluorometric | | Sensitivity: NO2-/NO3-= 10 nM | | Assay Time: 3-5 h | | Sample Type: Aqueous solutions | | Kit Contents: Assay Buffer, Nitrate Reductase, Enzyme Cofactors, Nitrate Standard, Nitrite Standard, DAN Reagent, NaOH, Microtiter Plates, Plate Covers, and a user protocol. | | Comments: Assay kit useful for the rapid quantitative measurement of nitric oxide (NO). Displays 50-fold increased sensitivity over the colorimetric nitric oxide assay kit (Cat. No. 482650). The assay is based on the enzymatic conversion of nitrate to nitrite by nitrate reductase, followed by the addition of 2,3-diaminonapthalene (DAN), and NaOH, which converts nitrite to a fluorescent compound. Fluorescence measurements of this compound accurately determine the nitrite (NO2-) concentration (excitation max.: 365 nm; emission max.: 450 nm). Do not use with nitrate- or nitrite-containing tissue culture media such as RPMI. Suitable for up to 80 tests. | | Ref.: Miles, A.M., et al. 1996. Methods Enzymol. 268, 105. Misko, T.P., et al. 1993. Anal. Biochem. 214, 11. Green, L.C., et al. 1982. Anal. Biochem. 126, 131. | | R: 34-36/37/38-45; S: 26-36/37/39-45-53 |
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