NOS Assay Kit

Format: Scintillation vial

Form: 50 Tests

Detection Method: Radiometric

Sensitivity: picomolar range

Assay Range: 2 h

Sample Type: Tissue or cell extracts

Kit Contents: Homogenization Buffer, Reaction Buffer, NADPH, CaCl2, Calmodulin, Stop Solution, Rat Brain Cerebellum Extract, NG-Nitro-L-Arginine Methyl Ester (L-NAME), Equilibrated Resin, Spin Cups, Cup Holders, and a user protocol. Radioactive arginine is not provided with this kit.

Comments: Assay kit useful for the rapid quantitative measurement of nitric oxide synthase (NOS) activity. It is based on the conversion of radioactive L-arginine to L-citrulline by NOS. Radioactive citrulline is separated from unreacted arginine on a cation-exchange resin and quantitated. Note: It is necessary to check the radiopurity of 3H-arginine before proceeding to use the kit.

Ref.: Bredt, D.S., and Snyder, S.H. 1994. Annu. Rev. Biochem. 63, 175. Kobzik, L., et al. 1994. Nature 372, 546. Moncada, S., and Higgs, A. 1994. N. Engl. J. Med. 329, 2002. Nathan, C., and Xie, Q.W. 1994. J. Biol. Chem. 269, 13725. Busconi, L., and Michel, T. 1993. J. Biol. Chem. 268, 8410. Marletta, M.A. 1993. J. Biol. Chem. 268, 12231. Schmidt, H.H., et al. 1993. Biochim. Biophys. Acta 1178, 153. Ignarro, L.J. 1991. Biochem. Pharmacol. 41, 485. Pollock, J.S., et al. 1991. Proc. Natl. Acad. Sci. U.S.A 88, 10480. Schmidt, H.H., et al. 1991. Proc. Natl. Acad. Sci. U.S.A 88, 365. Slater, M., et al. 1991. FEBS Lett. 291, 145. Stuehr, D.J., et al. 1991. Proc. Natl. Acad. Sci. U.S.A 88, 7773. Bredt, D.S., and Snyder, S.H. 1990. Proc. Natl. Acad. Sci. USA 87, 689. Bredt, D.S., and Snyder, S.H. 1989. Proc. Natl. Acad. Sci. USA 86, 9030. Knowles, R.G., et al. 1989. Proc. Natl. Acad. Sci. U.S.A 86, 5159.

R: 22-36/37/38; S: 26-36