NOS Assay Kit, Colorimetric

Format: 96-well plate

Form: 96 Tests

Detection Method: Colorimetric

Assay Time: 2 h

Sample Type: Plasma, serum, urine, tissue culture medium, and tissue homogenates

Kit Contents: Assay Buffer, Nitrate Reductase, Cofactors, Nitrate Standard, Lactate Dehydrogenase, Griess Reagents (R1 and R2), Microtiter Plate, Plate Cover, and a user protocol.

Comments: Assay kit useful for the rapid and accurate measurement of nitric oxide synthase (NOS) activity. This colorimetric assay uses lactate dehydrogenase (LDH) to destroy excess NADPH (cofactor for NOS) which interferes with the chemistry of Griess Reagent, commonly used for nitrite/nitrate detection. Do not use with nitrate- or nitrite-containing tissue culture media such as RPMI.

Ref.: Nims, R.W., et al. 1995. Methods Enzymol. 7, 48. Moncada, S. 1992. Acta Physiol. Scand. 145, 201. Nathan, C. 1992. FASEB J. 6, 3051. Green, L.C., et al. 1982. Anal. Biochem. 126, 131.