Format: Cuvette or 2 ml, 96-deep-well plate

Form: 500 Tests

Detection Method: Colorimetric

Kit Contents: UPPA™ Reagents I and II, Copper Solution I, Color Agents A and B, BSA Standard, and a user protocol.

Comments: An easy-to-use protein assay that overcomes interference of agents found in protein solutions including detergents, chelating agents, reducing agents, amines, sugars, urea, and others. The Universal Protein Precipitating Agent (UPPA™ Reagent) is used to precipitate and immobilize the protein in the tube while the interfering reagents are removed. Protein concentration is based on the specific binding of copper to the peptide backbone. As the protein concentration increases, the concentration of unbound copper ions decreases, and the color density is inversely related to the amount of protein present in solution.

Ref.: Ho, T.H. et al. 2005. Human Molecular Genetics 14, 1539. Ladd, A.N. et al. 2001. Mol. Cell. Biol. 21, 1285. Loeb, D.M. et al. 2002. J. Biol. Chem. 277, 19627. Shiels, A. et al. 2007. Invest. Ophthalmol. Vis. Sci. 48, 500. Gushwa, N.N. et al. 2003. Plant Physiology 132, 1925. DePinto, W. et al. 2006. Mol. Cancer Ther. 5, 2644 Werner, M.E. et al. 2007. J. Biol. Chem. 282, 5560. Dennison, S.M. et al (2006). Biophysical Journal 90, 1661. Reeve, I. et al. 2002. PNAS 99, 8608.

R: 36/37/38; S: 26-36