BGP
Bone-Gla-Protein Liquid. In 75 mM NaCl, 10 mM sodium phosphate buffer, pH 7.4. AVOID FREEZE/THAW CYCLES. Single chain vitamin K-dependent protein produced by osteoblasts. Osteocalcin purified from bovine bone. Binds to phospholipid vesicles in the presence of Ca2+ (Kd = 6 µM). Also binds hydroxylapatite. A good marker of bone turnover. May play a role in bone mineralization, bone resorption, and bone formation. Purity: ≥98% by SDS-PAGE. pI 4.0 - 4.5. H-Tyr-Leu-Asp-His-Trp-Leu-Gly-Ala-Pro-Ala-Pro-Tyr-Pro-Asp-Pro-Leu-Gla-Pro-Lys-Arg-Gla-Val-Cys23-Gla-Leu-Asn-Pro-Asp-Cys29-Asp-Glu-Leu-Ala-Asp-His-Ile-Gly-Phe-Gln-Glu-Ala-Tyr-Arg-Arg-Phe-Tyr-Gly-Pro-Val-OH (disulfide bond: 23 → 29) Ref.: Carter, S.D., et al. 1996. J. Anim. Sci. 74, 2719. Watson, K.E., et al. 1994. J. Clin. Invest. 93, 2106. Tracy, R.P., et al. 1990. J. Bone Min. Res. 5, 451. Gendreau, M.A., et al. 1989. J. Biol. Chem. 264, 6972. |
