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| | PhosphoDetect™ Anti-p38 MAP Kinase (pThr180, pTyr182) Rabbit pAb |
|  | Cat. No. 506119 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Thr180 and Tyr182 phosphorylation sites of human p38 MAP Kinase | | Form: Liquid | | Formulation: In Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3. | | Preservative: ≤0.1% sodium azide | | Positive Control: UV irradiated HEK293 cells or sorbitol-treated PC12 cells | | Comments: Recognizes p38a, b, and g/ERK6 phosphorylated at Thr180 and Tyr182 in uv-treated HEK293 cells and sorbitol-treated PC12 cells. | | Ref.: Marinissen, M.J., et al. 2001. Genes Dev. 15, 535. Nakagami, H., et al. 2001. Diabetes 50, 1472. Wilsbacher, J.L., et al. 1999. J. Biol. Chem. 274, 16988. Raingeaud, J., et al. 1995. J. Biol. Chem. 270, 7420. Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 10531. Han, J., et al. 1994. Science 265, 808. Lee, J.C., et al. 1994. Nature 372, 739. Rouse, J., et al. 1994. Cell 78, 1027. |
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EMD Chemicals Inc. USD list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
| | canine, human, mouse, rat | IB, IC
See Key |
Detection of human p38 MAP kinase phosphorylated at Thr180/Tyr182, by immunoblotting. Sample: Whole cell lysate from HEK293 cells treated with UV irradiation. Primary antibody: PhosphoDetect™ Anti-p38 MAP Kinase (pThr180, pTyr182) Rabbit pAb (Cat. No. 506119) (1:1000) pre-incubated with peptide immunogen (lane 1), a generic phophothreonine-containing peptide (lane 2), a generic phosphotyrosine-containg peptide (lane 3), non-phosphorylated peptide corresponding to the phosphopeptide (lane 4), without peptide (lane 5), with phophopeptide derived from the corresponding region of JNK1&2 (lane 6), and phosphopetide derived from the corresponding region of ERK1&2 (lane 7). Detection: chemiluminescence.
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